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FLuc-C-HiBiT
(Plasmid #207118)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207118 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21a (+)
  • Backbone manufacturer
    MilliporeSigma (Novagen)
  • Backbone size w/o insert (bp) 5370
  • Total vector size (bp) 7164
  • Vector type
    Bacterial Expression
  • Selectable markers
    N/A

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    luciferin 4-monooxygenase
  • Alt name
    FLuc_N_HiBiT
  • Alt name
    HiBiT-FLuc
  • Alt name
    Photinus pyralis luciferase
  • Species
    P. pyralis
  • Insert Size (bp)
    1760
  • Mutation
    Codon optmization for expression in BL21DE3
  • GenBank ID
    M15077.1
  • Promoter T7 Promotor
  • Tags / Fusion Proteins
    • HiBiT Tag (C terminal on insert)
    • His-tag with thrombin site (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (unknown if destroyed)
  • 3′ cloning site Xho1 (unknown if destroyed)
  • 5′ sequencing primer T7 promotor primer #69348-3
  • 3′ sequencing primer T7 terminator primer #69337-3
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    synthesized by Bio Basic for us.

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FLuc-C-HiBiT was a gift from James Inglese (Addgene plasmid # 207118 ; http://n2t.net/addgene:207118 ; RRID:Addgene_207118)

  • For your References section:

    A general assay platform to study protein pharmacology using ligand-dependent structural dynamics. Ciulla DA, Dranchak PK, Aitha M, van Neer RHP, Shah D, Tharakan R, Wilson KM, Wang Y, Braisted JC, Inglese J. Nat Commun. 2025 May 10;16(1):4342. doi: 10.1038/s41467-025-59658-6. 10.1038/s41467-025-59658-6 PubMed 40346061
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