Skip to main content
Addgene

DHFR-C-HiBiT
(Plasmid #207119)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207119 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    pET21a (+)
  • Backbone manufacturer
    EMD Biosciences (Novagen)
  • Backbone size w/o insert (bp) 5443
  • Total vector size (bp) 6063
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    None
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Human-dihydrofolate reductase-HiBiT-10xHis
  • Alt name
    Hs- DHFR-HiBiT-10xHis
  • Alt name
    DHFR-C-HiBiT
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    639
  • Mutation
    Codon optmization for expression in BL21DE3
  • GenBank ID
    1719 NCBI Gene ID 1719
  • Entrez Gene
    DHFR (a.k.a. DHFR1, DHFRP1, DYR)
  • Promoter T7
  • Tags / Fusion Proteins
    • HiBiT tag (C terminal on insert)
    • 10xHis-tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (unknown if destroyed)
  • 3′ cloning site Hind3 (unknown if destroyed)
  • 5′ sequencing primer T7 promotor primer #69348-3
  • 3′ sequencing primer T7 terminator primer #69337-3
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    DHFR-HiBiT-10xHis10 insert was synthsized for us by Bio Basic

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    DHFR-C-HiBiT was a gift from James Inglese (Addgene plasmid # 207119 ; http://n2t.net/addgene:207119 ; RRID:Addgene_207119)

  • For your References section:

    A general assay platform to study protein pharmacology using ligand-dependent structural dynamics. Ciulla DA, Dranchak PK, Aitha M, van Neer RHP, Shah D, Tharakan R, Wilson KM, Wang Y, Braisted JC, Inglese J. Nat Commun. 2025 May 10;16(1):4342. doi: 10.1038/s41467-025-59658-6. 10.1038/s41467-025-59658-6 PubMed 40346061
Related items: