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Ce iPGM-C-HiBiT
(Plasmid #207120)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207120 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21a (+)
  • Backbone manufacturer
    EMD Biosciences (Novagen)
  • Backbone size w/o insert (bp) 5443
  • Total vector size (bp) 7119
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    None
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    C. elegans-iPGM HiBIT His10
  • Alt name
    Ce iPGM-HiBiT-10xHis
  • Alt name
    ipgm-1
  • Species
    C. elegans (nematode)
  • Insert Size (bp)
    1737
  • Mutation
    Codon optmization for expression in BL21DE3
  • GenBank ID
    NP_491896.1
  • Entrez Gene
    ipgm-1 (a.k.a. CELE_F57B10.3)
  • Promoter T7 Promotor
  • Tags / Fusion Proteins
    • HiBiT Tag (C terminal on insert)
    • Thrombin cleavage site + 10x His Tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamH1 (unknown if destroyed)
  • 3′ cloning site Hind3 (unknown if destroyed)
  • 5′ sequencing primer T7 promotor primer #69348-3
  • 3′ sequencing primer T7 terminator primer #69337-3
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Ce-iPGM-10xHis_pET21a+ was provided by New England Biolabs and reported in a. Yu H, Dranchak P, Li Z, MacArthur R, Munson MS, Mehzabeen, N, Baird NJ, Battaile KP, Ross D, Lovell S, Carlow CKS, Suga H, Inglese J (2017) Macrocycle peptides delineate locked-open inhibition mechanism for microorganism phosphoglycerate mutases. Nat. Commun., 8: 14932, DOI .org/10.1038/ncomms14932

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Ce iPGM-C-HiBiT was a gift from James Inglese (Addgene plasmid # 207120 ; http://n2t.net/addgene:207120 ; RRID:Addgene_207120)

  • For your References section:

    A general assay platform to study protein pharmacology using ligand-dependent structural dynamics. Ciulla DA, Dranchak PK, Aitha M, van Neer RHP, Shah D, Tharakan R, Wilson KM, Wang Y, Braisted JC, Inglese J. Nat Commun. 2025 May 10;16(1):4342. doi: 10.1038/s41467-025-59658-6. 10.1038/s41467-025-59658-6 PubMed 40346061
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