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ABL1-N-HiBiT kinase domain_v1
(Plasmid #207121)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207121 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pET21a (+)
  • Backbone manufacturer
    MilliporeSigma (Novagen)
  • Backbone size w/o insert (bp) 5370
  • Total vector size (bp) 6324
  • Modifications to backbone
    N/A
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Human Abelson Kinase Domain N-terminal HiBiT, N-terminal 10X His-tag
  • Alt name
    ABL_N_HiBiT
  • Alt name
    Homo sapiens ABL proto-oncogene 1, non-receptor tyrosine kinase
  • Species
    H. sapiens (human)
  • Mutation
    Codon optmization for expression in BL21DE3
  • GenBank ID
    NG_012034.1
  • Entrez Gene
    ABL1 (a.k.a. ABL, BCR-ABL, CHDSKM, JTK7, bcr/abl, c-ABL, c-ABL1, p150, v-abl)
  • Promoter T7 Promotor
  • Tags / Fusion Proteins
    • HiBiT Tag (N terminal on insert)
    • His-tag with thrombin site (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Xho1 (unknown if destroyed)
  • 3′ cloning site BamH1 (unknown if destroyed)
  • 5′ sequencing primer T7 promotor primer #69348-3
  • 3′ sequencing primer T7 terminator primer #69337-3
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    synthsized by Bio Basic for us

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ABL1-N-HiBiT kinase domain_v1 was a gift from James Inglese (Addgene plasmid # 207121 ; http://n2t.net/addgene:207121 ; RRID:Addgene_207121)

  • For your References section:

    A general assay platform to study protein pharmacology using ligand-dependent structural dynamics. Ciulla DA, Dranchak PK, Aitha M, van Neer RHP, Shah D, Tharakan R, Wilson KM, Wang Y, Braisted JC, Inglese J. Nat Commun. 2025 May 10;16(1):4342. doi: 10.1038/s41467-025-59658-6. 10.1038/s41467-025-59658-6 PubMed 40346061
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