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pdTCas9_scr
(Plasmid #207568)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 207568 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pACYC184
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Plac/tet_dThermoCas9_PlacI_lacI_PJ23119_sgRNA(scrambled non-targeting spacer)
  • gRNA/shRNA sequence
    CTAGATCCGCAGTAACCCCATGGGTCATAGTTCCCCTGAGATTATCGCTGTGGTATAATGAAAGTTATACCACAGCAATGATCTCAGGGTTACTATGATAAGGGCTTTCTGCCTATAGGCAGACTGACCCGTGGCGTTGGGGATCGCCTATCGCCCGCTTTCTTCGGGCATTCCCCACTCTTAGGCG
  • Species
    Geobacillus thermodenitrificans T12 (thermocas9)
  • Mutation
    ThermoCas9(D8A,H582A)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pdTCas9_scr was a gift from John van der Oost (Addgene plasmid # 207568 ; http://n2t.net/addgene:207568 ; RRID:Addgene_207568)

  • For your References section:

    Characterization of the AcrIIC1 anti‒CRISPR protein for Cas9‒based genome engineering in E. coli. Trasanidou D, Potocnik A, Barendse P, Mohanraju P, Bouzetos E, Karpouzis E, Desmet A, van Kranenburg R, van der Oost J, Staals RHJ, Mougiakos I. Commun Biol. 2023 Oct 13;6(1):1042. doi: 10.1038/s42003-023-05418-5. 10.1038/s42003-023-05418-5 PubMed 37833505
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