HA-MDFIC
(Plasmid #207835)

• Purpose: Tagged MDFIC for western blotting and imaging
• Depositing Lab: Charles Cox
• Publication: Zhou et al Science. 2023 Aug 18;381(6659):799-804. doi: 10.1126/science.adh8190. Epub 2023 Aug 17.

Backbone

• Vector backbone: pcDNA3.1(-)
• Backbone size w/o insert (bp): 5000
• Total vector size (bp): 7531
• Modifications to backbone: Added IRES GFP
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: MDFIC
• Species: H. sapiens (human)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: NheI (not destroyed)
• 3′ cloning site: NotI (not destroyed)
• 5′ sequencing primer: CMV

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Codon optimized gBlock purchased from IDT

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry