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RGB-S reporter
(Plasmid #207841)


Item Catalog # Description Quantity Price (USD)
Plasmid 207841 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    GeneArt® Gene Synthesis, ThermoFisher Scientific and IDT Inc.
  • Backbone size w/o insert (bp) 4531
  • Total vector size (bp) 5409
  • Modifications to backbone
    PsulA::GFPmut3b::terminator_1 and PosmY::mRFP1::terminator_2 were integrated into pMK-RQ by GeneArt, then integration of terminator_3::PgrpE::mTagBFP2::terminator_4
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    Low Copy

Gene/Insert 1

  • Gene/Insert name
    RpoH sensing construct
  • Alt name
  • Species
  • Insert Size (bp)
  • GenBank ID
  • Promoter grpE

Cloning Information for Gene/Insert 1

  • Cloning method Gibson Cloning
  • 5′ sequencing primer CCAGTGAGCGCGACGTAATA
  • 3′ sequencing primer CGGCCACTCAACCCTATCTC
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    SOS sensing construct
  • Alt name
  • Species
  • Insert Size (bp)
  • GenBank ID
  • Promoter PsulA

Cloning Information for Gene/Insert 2

Gene/Insert 3

  • Gene/Insert name
    RpoS sensing construct
  • Alt name
  • Insert Size (bp)
  • GenBank ID
  • Promoter PosmY

Cloning Information for Gene/Insert 3

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The submitted plasmid version has additional 111 bp (1181-1291 bp) in the plasmid backbone compared to the version in the publication. The deposited plasmid has been tested and experimentally validated entailing no impact on the biosensor function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    RGB-S reporter was a gift from Christof Niemeyer (Addgene plasmid # 207841 ; ; RRID:Addgene_207841)
  • For your References section:

    A three-colour stress biosensor reveals multimodal response in single cells and spatiotemporal dynamics of biofilms. Zoheir AE, Sobol MS, Meisch L, Ordonez-Rueda D, Kaster AK, Niemeyer CM, Rabe KS. NPJ Biofilms Microbiomes. 2023 Aug 21;9(1):57. doi: 10.1038/s41522-023-00424-1. 10.1038/s41522-023-00424-1 PubMed 37604827