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(Plasmid #207959)


Item Catalog # Description Quantity Price (USD)
Plasmid 207959 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    A kind gift from Maria Vartiainen, University of Helsinki, Helsinki, Finland
  • Backbone size w/o insert (bp) 7839
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
  • Alt name
  • Species
    H. sapiens (human)
  • Insert Size (bp)
  • GenBank ID
  • Entrez Gene
    ARHGEF2 (a.k.a. GEF, GEF-H1, GEFH1, LFP40, Lfc, NEDMHM, P40)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (N terminal on backbone)

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The entry clone pENTR221-ARHGEF2 was from the ORFeome library at the Genome Biology Unit core facility, supported by HiLIFE and the Faculty of Medicine, University of Helsinki, and Biocenter Finland. This was used for the LR reaction with the pDEST-N-term_mCherry to generate the submitted plasmid, pDEST-mCherry-ARHGEF2.

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDEST-mCherry-ARHGEF2 was a gift from Johanna Ivaska (Addgene plasmid # 207959 ; ; RRID:Addgene_207959)
  • For your References section:

    Defined extracellular matrix compositions support stiffness-insensitive cell spreading and adhesion signaling. Conway JRW, Isomursu A, Follain G, Harma V, Jou-Olle E, Pasquier N, Valimaki EPO, Rantala JK, Ivaska J. Proc Natl Acad Sci U S A. 2023 Oct 24;120(43):e2304288120. doi: 10.1073/pnas.2304288120. Epub 2023 Oct 16. 10.1073/pnas.2304288120 PubMed 37844244