pSC101-GFPmut2-mScarlet-I C3T
(Plasmid #208195)

• Purpose: frameshift mistranslation, C3T
• Depositing Lab: Tanel Tenson
• Publication: Hinnu et al Sci Rep. 2024 Mar 22;14(1):6883. doi: 10.1038/s41598-024-57597-8.

Backbone

• Vector backbone: pSC101
• Backbone size w/o insert (bp): 3968
• Total vector size (bp): 5626
• Vector type: Bacterial Expression ; reporter

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: GFPmut2
• Species: Synthetic
• Insert Size (bp): 725
• GenBank ID:
• Promoter: Ptet+dnaK P1

Cloning Information for Gene/Insert 1

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: ctcatgtttgacagcttatcatcgataagc

Gene/Insert 2

• Gene/Insert name: mScarlet-I
• Species: Synthetic
• Insert Size (bp): 702
• Mutation: Frameshift insertion 6CCCT
• GenBank ID:
• Promoter: Ptet+dnaK P1

Cloning Information for Gene/Insert 2

• Cloning method: Ligation Independent Cloning
• 5′ sequencing primer: ACATGGTCCTTCTTGAGTTTGTAACAGC

Resource Information

• Supplemental Documents:
  • pSC101-GFPmut2-mScarlet-I C3T.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry