Addgene: Fluorescent reporters give new insights into antibiotics-induced nonsense and frameshift mistranslation.

This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Browse
Tanel Tenson
Hinnu et al

Fluorescent reporters give new insights into antibiotics-induced nonsense and frameshift mistranslation.
Hinnu M, Putrins M, Kogermann K, Kaldalu N, Tenson T
Sci Rep. 2024 Mar 22;14(1):6883. doi: 10.1038/s41598-024-57597-8. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
208183	pSC101-GFPmut2-mScarlet-I	mistranslation GFP and mScarlet-I positive control
208184	pSC101-AmpR EV	empty plasmid vector, non-fluorescent
208185	pSC101-GFPmut2(ΔmScarlet)	GFP positive, mScarlet-I negative control
208186	pSC101-GFPmut2-mScarlet-I 6Trp7Ser	mistranslation GFP and mScarlet-I positive control with Trp insertion
208187	pSC101-GFPmut2-mScarlet-I TAA	nonsense mistranslation, TAA stop
208188	pSC101-GFPmut2-mScarlet-I TAG	nonsense mistranslation, TAG stop
208189	pSC101-GFPmut2-mScarlet-I TGA	nonsense mistranslation, TGA stop
208190	pSC101-GFPmut2-mScarlet-I TGATC	nonsense mistranslation, TGA stop variant 2
208191	pSC101-GFPmut2-mScarlet-I T7	frameshift mistranslation, T7
208192	pSC101-GFPmut2-mScarlet-I A5	frameshift mistranslation, A5
208193	pSC101-GFPmut2-mScarlet-I A4	frameshift mistranslation, A4
208194	pSC101-GFPmut2-mScarlet-I A6T	frameshift mistranslation, A6T
208195	pSC101-GFPmut2-mScarlet-I C3T	frameshift mistranslation, C3T

Antibodies from Article