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Fluorescent reporters give new insights into antibiotics-induced nonsense and frameshift mistranslation.
Hinnu M, Putrins M, Kogermann K, Kaldalu N, Tenson T
Sci Rep. 2024 Mar 22;14(1):6883. doi: 10.1038/s41598-024-57597-8.
PubMed Article

Plasmids from Article

ID Plasmid Purpose
208183pSC101-GFPmut2-mScarlet-Imistranslation GFP and mScarlet-I positive control
208184pSC101-AmpR EVempty plasmid vector, non-fluorescent
208185pSC101-GFPmut2(ΔmScarlet)GFP positive, mScarlet-I negative control
208186pSC101-GFPmut2-mScarlet-I 6Trp7Sermistranslation GFP and mScarlet-I positive control with Trp insertion
208187pSC101-GFPmut2-mScarlet-I TAAnonsense mistranslation, TAA stop
208188pSC101-GFPmut2-mScarlet-I TAGnonsense mistranslation, TAG stop
208189pSC101-GFPmut2-mScarlet-I TGAnonsense mistranslation, TGA stop
208190pSC101-GFPmut2-mScarlet-I TGATCnonsense mistranslation, TGA stop variant 2
208191pSC101-GFPmut2-mScarlet-I T7frameshift mistranslation, T7
208192pSC101-GFPmut2-mScarlet-I A5frameshift mistranslation, A5
208193pSC101-GFPmut2-mScarlet-I A4frameshift mistranslation, A4
208194pSC101-GFPmut2-mScarlet-I A6Tframeshift mistranslation, A6T
208195pSC101-GFPmut2-mScarlet-I C3Tframeshift mistranslation, C3T

Antibodies from Article