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pLenti-sgStuffer-mCherry-Puro
(Plasmid #208350)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208350 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    lentiCRISPR v2
  • Backbone size (bp) 7615
  • Modifications to backbone
    Cas9 and BlastR were excised and mCherry was added via a combination of restriction and assembly cloning using lentiCRISPRv2 (Addgene 52961) as the starting backbone.
  • Vector type
    Lentiviral, CRISPR
  • Promoter hU6

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Construct was cloned by Mr. Phillip D. Corrin and Dr. Michael D. Nyquist. 4.7kb LTR-to-LTR with sgRNA inserted.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLenti-sgStuffer-mCherry-Puro was a gift from Peter S Nelson (Addgene plasmid # 208350 ; http://n2t.net/addgene:208350 ; RRID:Addgene_208350)

  • For your References section:

    Molecular consequences of acute versus chronic CDK12 loss in prostate carcinoma nominates distinct therapeutic strategies. Frank S, Persse T, Coleman I, Bankhead III A, Li D, De-Sarkar N, Wilson D, Rudoy D, Vashisth M, Galipeau P, Yang M, Hanratty B, Dumpit R, Morrissey C, Corey E, Montgomery RB, Haffner MC, Pritchard CC, Vasioukhin V, Ha G, Nelson PS. eLife 2025 10.7554/eLife.100081.2
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