CN2039-rAAV-3xSP10ins-eHGT_354h-minRho-SYFP2-WPRE3-BGHpA
(Plasmid
#208401)
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Purposeenhancer AAV for expression of SYFP2 in VIP+ cortical interneurons
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Depositing Labs
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 208401 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonerAAV
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Backbone manufacturercustom
- Backbone size w/o insert (bp) 4368
- Total vector size (bp) 5088
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Vector typeMammalian Expression, AAV, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameSYFP2
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SpeciesSynthetic
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Insert Size (bp)720
- Promoter eHGT_354h (enhancer)
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site MluI (not destroyed)
- 3′ cloning site SacI (not destroyed)
- 5′ sequencing primer n/a
- 3′ sequencing primer n/a (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made bySYFP2 insert was derived from Addgene plasmid#22905
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Allen Institute plasmid ALIAS: AiP12039
This work supported by NIH BRAIN Initiative grant UF1MH128339
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
CN2039-rAAV-3xSP10ins-eHGT_354h-minRho-SYFP2-WPRE3-BGHpA was a gift from The Allen Institute for Brain Science & Jonathan Ting (Addgene plasmid # 208401 ; http://n2t.net/addgene:208401 ; RRID:Addgene_208401) -
For your References section:
Signature morphoelectric properties of diverse GABAergic interneurons in the human neocortex. Lee BR, Dalley R, Miller JA, Chartrand T, Close J, Mann R, Mukora A, Ng L, Alfiler L, Baker K, Bertagnolli D, Brouner K, Casper T, Csajbok E, Donadio N, Driessens SLW, Egdorf T, Enstrom R, Galakhova AA, Gary A, Gelfand E, Goldy J, Hadley K, Heistek TS, Hill D, Hou WH, Johansen N, Jorstad N, Kim L, Kocsis AK, Kruse L, Kunst M, Leon G, Long B, Mallory M, Maxwell M, McGraw M, McMillen D, Melief EJ, Molnar G, Mortrud MT, Newman D, Nyhus J, Opitz-Araya X, Ozsvar A, Pham T, Pom A, Potekhina L, Rajanbabu R, Ruiz A, Sunkin SM, Szots I, Taskin N, Thyagarajan B, Tieu M, Trinh J, Vargas S, Vumbaco D, Waleboer F, Walling-Bell S, Weed N, Williams G, Wilson J, Yao S, Zhou T, Barzo P, Bakken T, Cobbs C, Dee N, Ellenbogen RG, Esposito L, Ferreira M, Gouwens NW, Grannan B, Gwinn RP, Hauptman JS, Hodge R, Jarsky T, Keene CD, Ko AL, Korshoej AR, Levi BP, Meier K, Ojemann JG, Patel A, Ruzevick J, Silbergeld DL, Smith K, Sorensen JC, Waters J, Zeng H, Berg J, Capogna M, Goriounova NA, Kalmbach B, de Kock CPJ, Mansvelder HD, Sorensen SA, Tamas G, Lein ES, Ting JT. Science. 2023 Oct 13;382(6667):eadf6484. doi: 10.1126/science.adf6484. Epub 2023 Oct 13. 10.1126/science.adf6484 PubMed 37824669
Map uploaded by the depositor.
