Skip to main content
Addgene
Open Menu

FUCGW-DEST
(Plasmid #208408)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208408 Standard format: Plasmid sent in bacteria as agar stab 1 $89
Add to Cart

Backbone

  • Vector backbone
    FUCGW
  • Backbone size (bp) 10576
  • Modifications to backbone
    A cassette containing attR1-CmR-ccDb-AttR2 was assembly cloned into the EcoRI site of FUCGW.
  • Vector type
    Lentiviral ; Gateway: DEST vector
  • Promoter UBC

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Ampicillin, 25 & 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    Unknown

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

FUCGW (PMID 16682621) was a gift from Dr. John K. Lee. FUCGW-DEST construct was cloned by Dr. Michael D. Nyquist.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    FUCGW-DEST was a gift from Peter S Nelson (Addgene plasmid # 208408 ; http://n2t.net/addgene:208408 ; RRID:Addgene_208408)

  • For your References section:

    Molecular consequences of acute versus chronic CDK12 loss in prostate carcinoma nominates distinct therapeutic strategies. Frank S, Persse T, Coleman I, Bankhead III A, Li D, De-Sarkar N, Wilson D, Rudoy D, Vashisth M, Galipeau P, Yang M, Hanratty B, Dumpit R, Morrissey C, Corey E, Montgomery RB, Haffner MC, Pritchard CC, Vasioukhin V, Ha G, Nelson PS. eLife 2025 10.7554/eLife.100081.2
Related items: