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pKLV2-U6gRNA5(hEGFR_1k)-PGKpuro2ABFP-W
(Plasmid #208431)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 208431 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pKLV2-U6gRNA5(BbsI)-PGKpuro2ABFP-W
  • Backbone size w/o insert (bp) 8648
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Selectable markers
    Puromycin ; BFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    EGFR
  • gRNA/shRNA sequence
    GCAAATAAAACCGGACTGA
  • Species
    H. sapiens (human)
  • Mutation
    N/A
  • Entrez Gene
    EGFR (a.k.a. ERBB, ERBB1, ERRP, HER1, NISBD2, NNCIS, PIG61, mENA)
  • Promoter human U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BbsI (destroyed during cloning)
  • 3′ cloning site BbsI (destroyed during cloning)
  • 5′ sequencing primer AGATAATTAGAATTAATTTGACTG
    • (Common Sequencing Primers)

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Used BbsI enzyme to clone in gRNA targeting gene of interest.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKLV2-U6gRNA5(hEGFR_1k)-PGKpuro2ABFP-W was a gift from Sok Ching Cheong (Addgene plasmid # 208431 ; http://n2t.net/addgene:208431 ; RRID:Addgene_208431)

  • For your References section:

    TNO155 is a selective SHP2 inhibitor to target PTPN11-dependent oral squamous cell carcinoma. Chai AWY, Tan YH, Ooi S, Yee PS, Yee SM, Cheong SC. Heliyon. 2024 Oct 22;10(21):e39677. doi: 10.1016/j.heliyon.2024.e39677. eCollection 2024 Nov 15. 10.1016/j.heliyon.2024.e39677 PubMed 39524880
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