pCFT7.2
(Plasmid #208808)

• Purpose: Expresses the fluorescent protein FAST
• Depositing Lab: François-Xavier Campbell-Valois
• Publication: Tchagang et al Front. Microbiol., 20 October 2023

Backbone

• Vector backbone: pUdO4a
• Backbone size w/o insert (bp): 2892
• Total vector size (bp): 3272
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: FAST
• Alt name: FAST1, Y-FAST
• Insert Size (bp): 378
• Promoter: tac

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: M13-rev
• 3′ sequencing primer: M13-fwd

Resource Information

• Supplemental Documents:
  • pcft7-2fast.gb
  • pcft7-2fast.gb

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry