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CMV-SynCas.v47 mammalian expression
(Plasmid #209003)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 209003 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pUC
  • Backbone size w/o insert (bp) 3368
  • Total vector size (bp) 7169
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SynCas.v47
  • Species
    Synthetic
  • Insert Size (bp)
    3861
  • Promoter CMV

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.02.23.581838 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CMV-SynCas.v47 mammalian expression was a gift from Omar Akbari (Addgene plasmid # 209003 ; http://n2t.net/addgene:209003 ; RRID:Addgene_209003)

  • For your References section:

    Synthetic Type III-E CRISPR-Cas Effectors for Programmable RNA-targeting. Brogan DJ, Lin CP, Benetta ED, Wang T, Chen F, Li H, Lin C, Komives EA, Akbari OS. J Mol Biol. 2026 Jan 15;438(2):169566. doi: 10.1016/j.jmb.2025.169566. Epub 2025 Nov 27. 10.1016/j.jmb.2025.169566 PubMed 41317788
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