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piggyBac-rtTA (4th_Gen)-sfGFP-IRES-NGN2-puro (VK_1122)
(Plasmid #209079)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 209079 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 209079-D50 50 μg of DNA in Tris buffer $495
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DNA 209079-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    piggyBac-rtTA (4th_Gen)
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    sfGFP
  • Species
    Synthetic
  • Promoter TRE4G

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    NGN2
  • Species
    M. musculus (mouse)
  • Entrez Gene
    Neurog2 (a.k.a. Atoh4, Math4A, bHLHa8, ngn-2, ngn2)

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

VK_1122

Please visit https://doi.org/10.1101/2022.11.08.515615 for bioRxiv preprint.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    piggyBac-rtTA (4th_Gen)-sfGFP-IRES-NGN2-puro (VK_1122) was a gift from Vikram Khurana (Addgene plasmid # 209079 ; http://n2t.net/addgene:209079 ; RRID:Addgene_209079)

  • For your References section:

    Rapid iPSC inclusionopathy models shed light on formation, consequence, and molecular subtype of alpha-synuclein inclusions. Lam I, Ndayisaba A, Lewis AJ, Fu Y, Sagredo GT, Kuzkina A, Zaccagnini L, Celikag M, Sandoe J, Sanz RL, Vahdatshoar A, Martin TD, Morshed N, Ichihashi T, Tripathi A, Ramalingam N, Oettgen-Suazo C, Bartels T, Boussouf M, Schabinger M, Hallacli E, Jiang X, Verma A, Tea C, Wang Z, Hakozaki H, Yu X, Hyles K, Park C, Wang X, Theunissen TW, Wang H, Jaenisch R, Lindquist S, Stevens B, Stefanova N, Wenning G, van de Berg WDJ, Luk KC, Sanchez-Pernaute R, Gomez-Esteban JC, Felsky D, Kiyota Y, Sahni N, Yi SS, Chung CY, Stahlberg H, Ferrer I, Schoneberg J, Elledge SJ, Dettmer U, Halliday GM, Bartels T, Khurana V. Neuron. 2024 Sep 4;112(17):2886-2909.e16. doi: 10.1016/j.neuron.2024.06.002. Epub 2024 Jul 29. 10.1016/j.neuron.2024.06.002 PubMed 39079530
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