pLCS100
(Plasmid
#209354)
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Purpose(Empty Backbone) Gateway cloning compatible vector for transient expression in protoplasts. Arabidopsis UBQ10 promoter expression of N-terminal YFP fusion protein.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 209354 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepUBQ10-YFP-GW
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Vector typePlant Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol and Ampicillin, 25 & 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.21203/rs.3.rs-3558280/v1 for preprint.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLCS100 was a gift from Lucia Strader (Addgene plasmid # 209354 ; http://n2t.net/addgene:209354 ; RRID:Addgene_209354) -
For your References section:
An improved toolkit of gateway- and gibson assembly-compatible vectors for protoplast transfection and agrobacterium-mediated plant transformation. Allen JR, Flanagan M, Pathak S, Emenecker SK, Emenecker RJ, Strader LC. BMC Res Notes. 2025 Apr 8;18(1):149. doi: 10.1186/s13104-025-07231-1. 10.1186/s13104-025-07231-1 PubMed 40200324
