pFA6 - iFAST
(Plasmid
#209414)
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PurposeiFAST containing plasmid for endogenous fusion constructs
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 209414 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepFA6
- Backbone size w/o insert (bp) 4589
- Total vector size (bp) 4967
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Vector typeYeast Expression
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Selectable markersNourseothricin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameiFAST
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SpeciesSynthetic
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Insert Size (bp)378
- Promoter none
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CTGAAGCTTCGTACGCTGCAATGGAACATGTTGCCTTTG
- 3′ sequencing primer CATGCATTTACTTATAATGGTTAAACCCTTTTGACAAACAC (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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Article Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pFA6 - iFAST was a gift from Patrick Van Dijck (Addgene plasmid # 209414 ; http://n2t.net/addgene:209414 ; RRID:Addgene_209414) -
For your References section:
A multi-colour fluorogenic tag and its application in Candida albicans. Devos J, Van Dijck P, Van Genechten W. Microbiology (Reading). 2024 Mar;170(3). doi: 10.1099/mic.0.001451. 10.1099/mic.0.001451 PubMed 38535895