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Addgene

pBTK1058
(Plasmid #209548)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 209548 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pBTK1001
  • Backbone size w/o insert (bp) 1895
  • Vector type
    Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Upstream homology arm for recombination into the Snodgrassella alvi wkB2 genome to replace clpA (SALWKB2_RS04465)
  • Species
    Snodgrassella alvi wkB2
  • Insert Size (bp)
    814

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BsmBI-v2 (destroyed during cloning)
  • 3′ cloning site BsmBI-v2 (destroyed during cloning)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.09.19.558440 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBTK1058 was a gift from Jeffrey Barrick (Addgene plasmid # 209548 ; http://n2t.net/addgene:209548 ; RRID:Addgene_209548)

  • For your References section:

    Single-step genome engineering in the bee gut symbiont Snodgrassella alvi. Lariviere PJ, Zuberi Ashraf AHM, Leonard SP, Miller LG, Moran NA, Barrick JE. bioRxiv 2023.09.19.558440 10.1101/2023.09.19.558440
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