pFPV-mCherry
(Plasmid #20956)

• Depositing Lab: Olivia Steele-Mortimer
• Publication: Drecktrah et al Traffic. 2008 Dec . 9(12):2117-29.

Backbone

• Vector backbone: pFPV
• Backbone size w/o insert (bp): 4000
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Growth instructions: DH10B
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: mCherry
• Species: Discosoma
• Insert Size (bp): 711
• GenBank ID: AY678264

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: XbaI (not destroyed)
• 3′ cloning site: SphI (not destroyed)
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: mCherry from Roger Tsien
• Articles Citing this Plasmid:
  • 20 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Shaner et al., (2004)
Nature Biotechnology 22,1567-1572