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p200-lib
(Plasmid #209615)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 209615 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    p200
  • Backbone size w/o insert (bp) 20
  • Total vector size (bp) 7529
  • Vector type
    CRISPR
  • Selectable markers
    EGFP

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    trans1-T1
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Bombyx mori gene
  • gRNA/shRNA sequence
    Bombyx mori gene
  • Species
    Bombyx mori
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site AarI (destroyed during cloning)
  • 3′ cloning site AarI (destroyed during cloning)
  • 5′ sequencing primer GTAAGACAAGGTCAGATAG
  • 3′ sequencing primer CGAGTCCTTTCCGATGTGT
  • (Common Sequencing Primers)
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p200-lib was a gift from Sanyuan Ma (Addgene plasmid # 209615)

  • For your References section:

    High-throughput and genome-scale targeted mutagenesis using CRISPR in a nonmodel multicellular organism, Bombyx mori. Ma S, Zhang T, Wang R, Wang P, Liu Y, Chang J, Wang A, Lan X, Sun L, Sun H, Shi R, Lu W, Liu D, Zhang N, Hu W, Wang X, Xing W, Jia L, Xia Q. Genome Res. 2024 Feb 7;34(1):134-144. doi: 10.1101/gr.278297.123. 10.1101/gr.278297.123 PubMed 38191205
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