pA-CBh.TP-CNOT6L-V5H6.MCh.Puro
(Plasmid #209945)

• Purpose: In mammalian cells expresses TP fused to a CNOT6L with a V5H6 tag. Also expresses an mCherry fluorescent protein and puromycin resistance marker.
• Depositing Lab: Kevin Weeks
• Publication: Plasmids for measuring proximity-mediated function of RNA regulatory proteins (unpublished)

Backbone

• Vector backbone: pA
• Total vector size (bp): 7509
• Vector type: Mammalian Expression
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Gene/Insert 1

• Gene/Insert name: CNOT6L
• Alt name: CCR4-NOT transcription complex subunit 6L
• Species: H. sapiens (human)
• Insert Size (bp): 1670
• Entrez Gene: CNOT6L (a.k.a. CCR4b)
• Promoter: CBh
• Tags / Fusion Proteins:
  • V5 epitope with H6 tag (C terminal on insert)
  • TP (MS2 coat protein) (N terminal on insert)

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: NA
• 3′ sequencing primer: NA

Gene/Insert 2

• Gene/Insert name: mCherry fluorescent protein fused to puromycin resistance marker
• Promoter: CMV

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: NA
• 3′ sequencing primer: NA

Resource Information

• Supplemental Documents:
  • pA-CBh.TP-CNOT6L-V5H6.MCh.Puro.gb
• A portion of this plasmid was derived from a plasmid made by: Vector builder and GeneWiz

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry