pNL3.2.CMV.NLuc-PEST.Hygro
(Plasmid
#209998)
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PurposeIn mammalian cells expresses NanoLuc mRNA with no TH as a control. Also expresses a hygromycin resistance marker.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 209998 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
Backbone
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Vector backbonepNL3.2
- Total vector size (bp) 5629
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Vector typeMammalian Expression
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Selectable markersHygromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameNanoLuc
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SpeciesSynthetic; derived from Oplophorus gracilirostris
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Insert Size (bp)639
- Promoter CMV
Cloning Information for Gene/Insert 1
- Cloning method Unknown
- 5′ sequencing primer NA
- 3′ sequencing primer NA (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameHygromycin resistance marker
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Insert Size (bp)1038
- Promoter SV40
Cloning Information for Gene/Insert 2
- Cloning method Unknown
- 5′ sequencing primer NA
- 3′ sequencing primer NA (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byPromega
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNL3.2.CMV.NLuc-PEST.Hygro was a gift from Kevin Weeks (Addgene plasmid # 209998 ; http://n2t.net/addgene:209998 ; RRID:Addgene_209998)
Map uploaded by the depositor.
