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Addgene

HOXD4-WV-MSCV
(Plasmid #21002)

Full plasmid sequence is not available for this item.

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 21002 Standard format: Plasmid sent in bacteria as agar stab 1 $85

Backbone

  • Vector backbone
    MSCV
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 6500
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    HOXD4
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1000
  • Mutation
    W->V PBX1 interaction mutant
  • Entrez Gene
    Hoxd4 (a.k.a. 6030436D05Rik, Hox-4.2, Hox-5.1)
  • Tags / Fusion Proteins
    • His (N terminal on insert)
    • T7-tag (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XbaI (blunted) (destroyed during cloning)
  • 3′ cloning site XbaI (blunted) (destroyed during cloning)
  • 5′ sequencing primer MSCV
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Mark Featherstone

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Mutation prevents interaction with PBX proteins

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    HOXD4-WV-MSCV was a gift from Corey Largman (Addgene plasmid # 21002 ; http://n2t.net/addgene:21002 ; RRID:Addgene_21002)