HOXD12 (murine) HIS-tag pET
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8553||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Insert Size (bp)838
MutationFusion sequence is: ggatccATG
Entrez GeneHoxd12 (a.k.a. Hox-4.7, Hox-5.6)
/ Fusion Proteins
- T7 (N terminal on backbone)
- His (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site BamHI (not destroyed)
- 3′ cloning site XhoI (destroyed during cloning)
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:HOXD12 (murine) HIS-tag pET was a gift from Corey Largman (Addgene plasmid # 8553 ; http://n2t.net/addgene:8553 ; RRID:Addgene_8553)
For your References section:AbdB-like Hox proteins stabilize DNA binding by the Meis1 homeodomain proteins. Shen WF, Montgomery JC, Rozenfeld S, Moskow JJ, Lawrence HJ, Buchberg AM, Largman C. Mol Cell Biol 1997 Nov;17(11):6448-58. PubMed 9343407
Map uploaded by the depositor.