HOXA11 (murine) HIS-tag pET
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8517||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Vector backbonepET 28a
- Backbone size w/o insert (bp) 5400
Vector typeBacterial Expression
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Insert Size (bp)2100
Mutationmakes protein by Western
Entrez GeneHoxa11 (a.k.a. Hox-1.9, Hoxa-11)
/ Fusion Protein
- T7 (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site HindIII (not destroyed)
- 5′ sequencing primer T7
- 3′ sequencing primer SP6 (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made bySteve Potter
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Clone contains the sequence: aag ccg cgc cca between the EcoR1 of pET and the ATG start
See author's map.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:HOXA11 (murine) HIS-tag pET was a gift from Corey Largman (Addgene plasmid # 8517 ; http://n2t.net/addgene:8517 ; RRID:Addgene_8517)
For your References section:AbdB-like Hox proteins stabilize DNA binding by the Meis1 homeodomain proteins. Shen WF, Montgomery JC, Rozenfeld S, Moskow JJ, Lawrence HJ, Buchberg AM, Largman C. Mol Cell Biol 1997 Nov;17(11):6448-58. PubMed 9343407
Map uploaded by the depositor.