HOXA2 (murine) HIS-tag pET
(Plasmid #8557)

• Depositing Lab: Corey Largman
• Publication: HOX protein coding clones not referenced in publications (unpublished)

Backbone

• Vector backbone: pET28a
• Backbone size w/o insert (bp): 5400
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: HOXA2
• Alt name: HOX1K
• Alt name: HOX1.11
• Species: M. musculus (mouse)
• Insert Size (bp): 1250
• Mutation: contains 5 additional amino acids from 5' untranslated sequence prior to ATG start; makes protein by Western
• GenBank ID: M93148
• Entrez Gene: Hoxa2 (a.k.a. HOX1.11, Hox-1.11)
• Tags / Fusion Proteins:
  • T7 (N terminal on backbone)
  • His (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: StyI/EcoRI blunt (not destroyed)
• 3′ cloning site: HindIII (not destroyed)
• 5′ sequencing primer: T7

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Marshall Nirenberg

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Tan, D.-P., Ferrante, J., Nazarali, A., Shao, X-P., Kozak, C. A.,, Guo, V., and Nirenberg, M. Proc. Natl. Acad. Sci. USA 89: 6280-6284, 1992