HOXA4 (murine) pET
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||8488||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5400
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
SpeciesM. musculus (mouse)
Insert Size (bp)856
MutationThr-2 changed to Ala-2 to form better ribosomal binding site
Entrez GeneHoxa4 (a.k.a. AV206827, Hox-1.4, MGC130055)
/ Fusion Proteins
- T7 (N terminal on backbone)
- His (N terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site EcoRI (not destroyed)
- 3′ cloning site XbaI/XhoI blunt (destroyed during cloning)
- 5′ sequencing primer T7 (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made byDeborah Wolgemuth
Terms and Licenses
improved ribosomal binding site:
TTC ACC ATG G
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:HOXA4 (murine) pET was a gift from Corey Largman (Addgene plasmid # 8488 ; http://n2t.net/addgene:8488 ; RRID:Addgene_8488)
For your References section:Hox homeodomain proteins exhibit selective complex stabilities with Pbx and DNA. Shen WF, Chang CP, Rozenfeld S, Sauvageau G, Humphries RK, Lu M, Lawrence HJ, Cleary ML, Largman C. Nucleic Acids Res 1996 Mar 1;24(5):898-906. 10.1093/nar/24.5.898 PubMed 8600458
Map uploaded by the depositor.