Skip to main content
Addgene

AAV-pCamKII-Cre-pU6-SpCas9gRNAentry
(Plasmid #210391)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 210391 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Add to Cart

Backbone

  • Vector backbone
    Addgene plasmid 137178
  • Vector type
    Mammalian Expression, AAV, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    AAV-(ITR)-pCamKII-NLS-Cre-WPRE-bGHpA-(rev)-SpCas9_gRNA-BsmBI_RFPcassette-hU6-(ITR) (NJA158)
  • Alt name
    NJA158
  • Species
    H. sapiens (human), M. musculus (mouse)
  • Promoter CamKII promoter for Cre, human U6 promoter for SpCas9 gRNA

Cloning Information

  • Cloning method Gibson Cloning
  • 5′ sequencing primer oBK33-GCAGCGTATCCACATAGCG
  • 3′ sequencing primer oBK1867-GGACTATCATATGCTTACCGTAACTTG
    • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    AAV-pCamKII-Cre-pU6-SpCas9gRNAentry was a gift from Benjamin Kleinstiver (Addgene plasmid # 210391 ; http://n2t.net/addgene:210391 ; RRID:Addgene_210391)

  • For your References section:

    Myocardial infarction augments sleep to limit cardiac inflammation and damage. Huynh P, Hoffmann JD, Gerhardt T, Kiss MG, Zuraikat FM, Cohen O, Wolfram C, Yates AG, Leunig A, Heiser M, Gaebel L, Gianeselli M, Goswami S, Khamhoung A, Downey J, Yoon S, Chen Z, Roudko V, Dawson T, Ferreira da Silva J, Ameral NJ, Morgenroth-Rebin J, D'Souza D, Koekkoek LL, Jacob W, Munitz J, Lee D, Fullard JF, van Leent MMT, Roussos P, Kim-Schulze S, Shah N, Kleinstiver BP, Swirski FK, Leistner D, St-Onge MP, McAlpine CS. Nature. 2024 Nov;635(8037):168-177. doi: 10.1038/s41586-024-08100-w. Epub 2024 Oct 30. 10.1038/s41586-024-08100-w PubMed 39478215
Related items: