pTIPI_mIGg2a_Anti-HA [12CA5]
(Plasmid
#210656)
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PurposeMammalian expression of Anti-HA [12CA5] mouse IgG2a heavy chain; to be used with pTIPI_mK_Anti-HA [12CA5] light chain (Plasmid 210657) to make the antibody.
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Depositing Labs
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Publication
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Sequence Information
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Sequences (2) — Accept Affinity Reagent Sequence Policy
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Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 210656 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 * |
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Backbone
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Vector backbonepTipi2.1
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Backbone manufacturerInstitute for Protein Innovation (IPI) (Addgene plasmid # 225877)
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Vector typeMammalian Expression, Affinity Reagent/ Antibody
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAnti-HA [12CA5] mouse IgG2a heavy chain
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SpeciesM. musculus (mouse)
- Promoter CMV
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Tag
/ Fusion Protein
- Mouse IgG2a heavy chain (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV
- 3′ sequencing primer WPRE (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byAntibody gene found in literature.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTIPI_mIGg2a_Anti-HA [12CA5] was a gift from Melina Fan & Addgene Research Program (Addgene plasmid # 210656 ; http://n2t.net/addgene:210656 ; RRID:Addgene_210656)