pTIPI_mK_Anti-HA [12CA5]
(Plasmid
#210657)
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PurposeMammalian expression of Anti-HA [12CA5] mouse kappa light chain; to be used with pTIPI_mIGg2a_Anti-HA [12CA5] heavy chain (Plasmid 210656) to make the antibody.
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Depositing Labs
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Publication
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Sequence Information
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Sequences (2) — Accept Affinity Reagent Sequence Policy
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Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 210657 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 * |
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Backbone
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Vector backbonepTipi2.1
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Backbone manufacturerInstitute for Protein Innovation (IPI) (Addgene plasmid # 225877)
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Vector typeMammalian Expression, Affinity Reagent/ Antibody
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameAnti-HA [12CA5] kappa light chain
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SpeciesM. musculus (mouse)
- Promoter CMV
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Tag
/ Fusion Protein
- Mouse kappa light chain (C terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV
- 3′ sequencing primer WPRE (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byAntibody gene found in literature.
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pTIPI_mK_Anti-HA [12CA5] was a gift from Melina Fan & Addgene Research Program (Addgene plasmid # 210657 ; http://n2t.net/addgene:210657 ; RRID:Addgene_210657)