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pQβEP1
(Plasmid #210859)

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This material is available to academics and nonprofits only. Currently unavailable outside the U.S.
Item Catalog # Description Quantity Price (USD)
Plasmid 210859 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pBR322
  • Backbone size w/o insert (bp) 450
  • Total vector size (bp) 7800
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    SARS-CoV Spike AA441-460
  • Entrez Gene
    S (a.k.a. sars2, E2)
  • Promoter T7
  • Tag / Fusion Protein
    • No

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Bpu 10I or Afl II (unknown if destroyed)
  • 3′ cloning site Nsi (unknown if destroyed)
  • 5′ sequencing primer CCA GGC ATA TGC TGA CGT GAC TTT TTC GTTCAC
  • 3′ sequencing primer TAGACATgcatttcatccttagGCGGCCGCTTACTAaaacggcacgttgctaatatcgcgttcaaacgggcgcagtttgccatggcgcagatagcGgccgccgctgccgccagcacgagg
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Not Applicable

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.07.27.550841 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pQβEP1 was a gift from Alain Bopda Waffo (Addgene plasmid # 210859 ; http://n2t.net/addgene:210859 ; RRID:Addgene_210859)

  • For your References section:

    Mapping immunological and host receptor binding determinants of SARS-CoV spike protein utilizing the Qubevirus platform. Sanders C, Dzelamonyuy A, Ntemafack A, Alatoom N, Nchinda G, Georgiadis MM, Bopda Waffo A. J Biol Chem. 2023 Dec;299(12):105460. doi: 10.1016/j.jbc.2023.105460. Epub 2023 Nov 15. 10.1016/j.jbc.2023.105460 PubMed 37977224
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