pNICBio2_WDR5:2-334
(Plasmid
#211027)
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Purposebacterial expression of human WDR5 fragment A2 to C334. N-terminal 10X His tag with TEV cleavage and C-terminal biotin acceptor peptide tag.
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 211027 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 * |
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Backbone
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Vector backbonepNICBio2
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Backbone manufacturerpET28a modified
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberLow Copy
Gene/Insert
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Gene/Insert nameWDR5:A2-C334
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SpeciesH. sapiens (human)
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Insert Size (bp)999
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Mutationwild type
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Entrez GeneWDR5 (a.k.a. BIG-3, BIG3, CFAP89, SWD3)
- Promoter T7
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Tags
/ Fusion Proteins
- MHHHHHHHHHHDLGTENLYFQS (N terminal on insert)
- SSKGGYGLNDIFEAQKIEWHE (C terminal on insert)
Cloning Information
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer T7
- 3′ sequencing primer T7term (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byMammalian Gene Collection (BC001635)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
5' Cloning Site: BsaI (destroyed), 3' Cloning Site: BsaI (destroyed).
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pNICBio2_WDR5:2-334 was a gift from Cheryl Arrowsmith (Addgene plasmid # 211027 ; http://n2t.net/addgene:211027 ; RRID:Addgene_211027) -
For your References section:
A Target Class Ligandability Evaluation of WD40 Repeat-Containing Proteins. Ackloo S, Li F, Szewczyk M, Seitova A, Loppnau P, Zeng H, Xu J, Ahmad S, Arnautova YA, Baghaie AJ, Beldar S, Bolotokova A, Centrella PA, Chau I, Clark MA, Cuozzo JW, Dehghani-Tafti S, Disch JS, Dong A, Dumas A, Feng JA, Ghiabi P, Gibson E, Gilmer J, Goldman B, Green SR, Guie MA, Guilinger JP, Harms N, Herasymenko O, Houliston S, Hutchinson A, Kearnes S, Keefe AD, Kimani SW, Kramer T, Kutera M, Kwak HA, Lento C, Li Y, Liu J, Loup J, Machado RAC, Mulhern CJ, Perveen S, Righetto GL, Riley P, Shrestha S, Sigel EA, Silva M, Sintchak MD, Slakman BL, Taylor RD, Thompson J, Torng W, Underkoffler C, von Rechenberg M, Walsh RT, Watson I, Wilson DJ, Wolf E, Yadav M, Yazdi AK, Zhang J, Zhang Y, Santhakumar V, Edwards AM, Barsyte-Lovejoy D, Schapira M, Brown PJ, Halabelian L, Arrowsmith CH. J Med Chem. 2025 Jan 23;68(2):1092-1112. doi: 10.1021/acs.jmedchem.4c02010. Epub 2024 Nov 4. 10.1021/acs.jmedchem.4c02010 PubMed 39495097