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pRK5R 99xGly
(Plasmid #211356)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 211356 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pRK5R
  • Modifications to backbone
    Reversed origin of replication versus pRK5.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    99xGly-EGFP-3xHA
  • Species
    H. sapiens (human)
  • Tag / Fusion Protein
    • EGFP-3xHA (C terminal on insert)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This material includes a long poly-glycine repeat that is challenging to maintain and sequence accurately. Addgene’s long-read sequencing results confirm approximately 94 repeats in the current samples. The depositor recommends that repeat length should be verified for each prep via restriction digest, Nanopore sequencing, or both. The depositing lab is happy to provide guidance if needed.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRK5R 99xGly was a gift from Raghu Chivukula (Addgene plasmid # 211356 ; http://n2t.net/addgene:211356 ; RRID:Addgene_211356)

  • For your References section:

    Polyglycine-mediated aggregation of FAM98B disrupts tRNA processing in GGC repeat disorders. Yang J, Xu Y, Ziehr DR, Taylor MS, Valenstein ML, Frenkel EM, Bush JR, Rutter K, Stevanovski I, Shi CY, Kesavan M, Pinto RM, Deveson I, Bartel DP, Sabatini DM, Chivukula RR. Science. 2025 Jul 17;389(6757):eado2403. doi: 10.1126/science.ado2403. Epub 2025 Jul 17. 10.1126/science.ado2403 PubMed 40674500
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