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Addgene

pTRNA scPhe-GAA
(Plasmid #211359)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 211359 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pTRNA
  • Modifications to backbone
    pRK5 backbone modified to express tRNA under control of H1 (Pol III) promoter.
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    S. cerevisiae Phe-GAA tRNA
  • Species
    S. cerevisiae (budding yeast)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTRNA scPhe-GAA was a gift from Raghu Chivukula (Addgene plasmid # 211359 ; http://n2t.net/addgene:211359 ; RRID:Addgene_211359)

  • For your References section:

    Polyglycine-mediated aggregation of FAM98B disrupts tRNA processing in GGC repeat disorders. Yang J, Xu Y, Ziehr DR, Taylor MS, Valenstein ML, Frenkel EM, Bush JR, Rutter K, Stevanovski I, Shi CY, Kesavan M, Pinto RM, Deveson I, Bartel DP, Sabatini DM, Chivukula RR. Science. 2025 Jul 17;389(6757):eado2403. doi: 10.1126/science.ado2403. Epub 2025 Jul 17. 10.1126/science.ado2403 PubMed 40674500
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