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ISDra2-TnpB-mCherry-U6-ωRNA*
(Plasmid #212967)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 212967 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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DNA 212967-D50 50 μg of DNA in Tris buffer $495
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DNA 212967-D100 100 μg of DNA in Tris buffer $585
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Backbone

  • Vector backbone
    unknown
  • Total vector size (bp) 8090
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    ISDra2-TnpB-T2A-mCherry
  • Species
    Synthetic; Deinococcus radiodurans
  • Promoter CAG, T7
  • Tags / Fusion Proteins
    • NLS (N terminal on insert)
    • NLS-FLAG (C terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site unknown (unknown if destroyed)
  • 3′ cloning site unknown (unknown if destroyed)
  • 5′ sequencing primer n/a
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    ωRNA*
  • Species
    Synthetic
  • Promoter U6

Cloning Information for Gene/Insert 2

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Xu Lab plasmid pCX2280.

Please visit https://doi.org/10.1101/2023.06.29.547137 for bioRxiv preprint.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 50 μg
  • Concentration 1 μg/μL
  • Pricing $495 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

Purpose

Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.

Delivery

  • Amount 100 μg
  • Concentration 1 μg/μL
  • Pricing $585 USD
  • Storage 4 ℃ (short-term) or -20 ℃ (long-term)

Terms and Licenses

Quality Control

Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.

Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    ISDra2-TnpB-mCherry-U6-ωRNA* was a gift from Chunlong Xu (Addgene plasmid # 212967 ; http://n2t.net/addgene:212967 ; RRID:Addgene_212967)

  • For your References section:

    Engineering a transposon-associated TnpB-omegaRNA system for efficient gene editing and phenotypic correction of a tyrosinaemia mouse model. Li Z, Guo R, Sun X, Li G, Shao Z, Huo X, Yang R, Liu X, Cao X, Zhang H, Zhang W, Zhang X, Ma S, Zhang M, Liu Y, Yao Y, Shi J, Yang H, Hu C, Zhou Y, Xu C. Nat Commun. 2024 Jan 27;15(1):831. doi: 10.1038/s41467-024-45197-z. 10.1038/s41467-024-45197-z PubMed 38280857
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