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Addgene

pU6-LbCpf1-crR2
(Plasmid #213047)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 213047 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pX330
  • Total vector size (bp) 3686
  • Vector type
    Mammalian Expression, CRISPR

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    crR2
  • Insert Size (bp)
    45
  • Promoter U6

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BbsI (destroyed during cloning)
  • 3′ cloning site BbsI (destroyed during cloning)
  • 5′ sequencing primer hU6-F
  • 3′ sequencing primer RVprimer3
    • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pU6-LbCpf1-crR2 was a gift from Petr Bartunek (Addgene plasmid # 213047 ; http://n2t.net/addgene:213047 ; RRID:Addgene_213047)

  • For your References section:

    SWITCHER, a CRISPR-inducible floxed wild-type Cre regulating CRISPR activity. Schuster B, Dobiasovska I, Curcic J, Pajer P, Borna S, Bartunek P. Commun Biol. 2025 Jul 2;8(1):982. doi: 10.1038/s42003-025-08427-8. 10.1038/s42003-025-08427-8 PubMed 40596718
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