pLoxP-EGFP-crRNA-entry
(Plasmid
#213048)
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PurposeSWITCHER ready LoxP-EGFP reporter plasmid for cloning of a customized CRISPR-inducible Cre-construct
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 213048 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
Backbone
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Vector backbonepX330
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Vector typeMammalian Expression, CRISPR
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameLoxP-EGFP-MALAT1-triplex
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Insert Size (bp)1145
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GenBank IDU55762
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7 (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLoxP-EGFP-crRNA-entry was a gift from Petr Bartunek (Addgene plasmid # 213048 ; http://n2t.net/addgene:213048 ; RRID:Addgene_213048) -
For your References section:
SWITCHER, a CRISPR-inducible floxed wild-type Cre regulating CRISPR activity. Schuster B, Dobiášovská I, Ćurčić J, Pajer P, Borna Š, Bartůněk P. Commun Biol 2025. 8, 982. doi: https://doi.org/10.1038/s42003-025-08427-8 10.1038/s42003-025-08427-8