pHTN_HaloTag_KIF5B[1-560]
(Plasmid
#213403)
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PurposeTruncated human kinesin-1 with HaloTag at the N-terminus
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Depositing Lab
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Depositing OrganizationEuropean Molecular Biology Laboratory (EMBL), Heidelberg
Located in Germany -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 213403 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 213403-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 213403-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonepHTN HaloTag CMV-neo
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Backbone manufacturerPromega
- Backbone size w/o insert (bp) 6143
- Total vector size (bp) 8959
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameHaloTag-KIF5B[1-560]
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Alt nameHuman kinesin-1[1-560]
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SpeciesH. sapiens (human)
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Insert Size (bp)2613
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GenBank ID3799
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Entrez GeneKIF5B (a.k.a. HEL-S-61, KINH, KNS, KNS1, UKHC)
- Promoter CMV
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Tag
/ Fusion Protein
- HaloTag (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV-F
- 3′ sequencing primer SV40pA-R
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
DNA (Catalog # 213403-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 213403-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHTN_HaloTag_KIF5B[1-560] was a gift from Jonas Ries (Addgene plasmid # 213403 ; http://n2t.net/addgene:213403 ; RRID:Addgene_213403) -
For your References section:
Direct observation of motor protein stepping in living cells using MINFLUX. Deguchi T, Iwanski MK, Schentarra EM, Heidebrecht C, Schmidt L, Heck J, Weihs T, Schnorrenberg S, Hoess P, Liu S, Chevyreva V, Noh KM, Kapitein LC, Ries J. Science. 2023 Mar 10;379(6636):1010-1015. doi: 10.1126/science.ade2676. Epub 2023 Mar 9. 10.1126/science.ade2676 PubMed 36893247
Map uploaded by the depositor.
