pCMV-Parkin-A92mKO2
(Plasmid
#213548)
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PurposeTransient mammalian expresssion of Parkin, internally tagged with mKO2
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Depositing Labs
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Depositing OrganizationUniversity of California, San Francisco (UCSF)
Located in United States of America -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 213548 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 213548-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 213548-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backboneAddgene #23956
- Backbone size w/o insert (bp) 3954
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Vector typeMammalian Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameParkin
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Alt namePARK2
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SpeciesH. sapiens (human)
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Insert Size (bp)2079
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Entrez GenePRKN (a.k.a. AR-JP, LPRS2, PARK2, PDJ)
- Promoter CMV
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer CMV Forward
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made bymKO2 from Addgene #83841
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Parkin Internally tagged with mKO2 following Alanine 92
DNA (Catalog # 213548-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 213548-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pCMV-Parkin-A92mKO2 was a gift from Steven Altschuler & Lani Wu (Addgene plasmid # 213548 ; http://n2t.net/addgene:213548 ; RRID:Addgene_213548) -
For your References section:
A PINK1 input threshold arises from positive feedback in the PINK1/Parkin mitophagy decision circuit. Waters CS, Angenent SB, Altschuler SJ, Wu LF. Cell Rep. 2023 Oct 31;42(10):113260. doi: 10.1016/j.celrep.2023.113260. Epub 2023 Oct 17. 10.1016/j.celrep.2023.113260 PubMed 37851575
