pAAV-P3-Alb-StayGold
(Plasmid #213790)

• Purpose: To produce AAV with the P3 promoter to express Alb(mouse)-StayGold fusion protein
• Depositing Lab: Hajime Hirase
• Publication: Knak et al Unpublished

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• Serotype: Select serotype for details See details about
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Backbone

• Vector backbone: pAAV-P3
• Backbone manufacturer: VVF (p438) https://vvf.ethz.ch/
• Backbone size w/o insert (bp): 4354
• Total vector size (bp): 6886
• Vector type: Mammalian Expression, AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Alb
• Alt name: Alb1 Alb-1
• Species: M. musculus (mouse)
• Entrez Gene: Alb (a.k.a. Alb-1, Alb1, BCL001, BCL002, BPL001)
• Tag / Fusion Protein:
  • StayGold (C terminal on insert)

Resource Information

• Supplemental Documents:
  • pAAV-P3-Alb1-StayGold-WPRE annotated 20231212a.gbk
• A portion of this plasmid was derived from a plasmid made by: Artificially synthesized the StayGold sequence from the published amino acid sequence by Hirano et al. (2022, NatBioTech).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Dim when AAV8 is injected systemically in C57BL/6 mice. Use pAAV-P3-Alb-mStayGold (Addgene #234549) for plasma expression (AAV8).