delCMV-mCherry-2x-Rhotekin-GBD
(Plasmid
#214142)
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PurposeRho activity sensor
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Depositing Lab
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Depositing OrganizationTechnische Universitaet Dortmund
Located in Germany -
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 214142 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
| DNA | 214142-D50 | 50 μg of DNA in Tris buffer | $495 | ||
| DNA | 214142-D100 | 100 μg of DNA in Tris buffer | $585 | ||
Backbone
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Vector backbonedelCMV-mCherry
- Total vector size (bp) 4776
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Vector typeMammalian Expression
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Selectable markersNeomycin (select with G418)
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert name3xRhotekin-GBD
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SpeciesM. musculus (mouse)
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Insert Size (bp)246
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MutationOnly the GTPase binding domain (GBD) is included
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Entrez GeneRtkn
- Promoter delCMV
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Tag
/ Fusion Protein
- mCherry (N terminal on backbone)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer whole plasmid sequencing
- (Common Sequencing Primers)
Resource Information
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A portion of this plasmid was derived from a plasmid made byRyohei Yasuda (Addgene plasmid # 29675)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
the delCMV promotor was derived from Watanabe and Mitchison, 2002, Science, 295:1083
DNA (Catalog # 214142-D50)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 50 μg
- Concentration 1 μg/μL
- Pricing $495 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
DNA (Catalog # 214142-D100)
Purpose
Ready-to-use, high-purity DNA prep. DNA aliquots are suitable for use in mammalian cell transfections and other molecular biology applications.
Delivery
- Amount 100 μg
- Concentration 1 μg/μL
- Pricing $585 USD
- Storage 4 ℃ (short-term) or -20 ℃ (long-term)
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Quality Control
Every DNA prep is verified by next-generation sequencing, confirming plasmid identity, sequence integrity, and absence of DNA contaminants. Concentration is confirmed to be within ±2.5% of the stated concentration.
Our production process has been validated to consistently yield endotoxin levels below 1.0 EU/μg. Individual lots are not tested for endotoxin.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
delCMV-mCherry-2x-Rhotekin-GBD was a gift from Leif Dehmelt (Addgene plasmid # 214142 ; http://n2t.net/addgene:214142 ; RRID:Addgene_214142) -
For your References section:
Rho GTPase activity crosstalk mediated by Arhgef11 and Arhgef12 coordinates cell protrusion-retraction cycles. Nanda S, Calderon A, Sachan A, Duong TT, Koch J, Xin X, Solouk-Stahlberg D, Wu YW, Nalbant P, Dehmelt L. Nat Commun. 2023 Dec 15;14(1):8356. doi: 10.1038/s41467-023-43875-y. 10.1038/s41467-023-43875-y PubMed 38102112
Map uploaded by the depositor.
