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pET-11a-pH-LeuRS_WT
(Plasmid #214193)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214193 Standard format: Plasmid sent in bacteria as agar stab 1 $94

Backbone

  • Vector backbone
    pET-11a
  • Backbone manufacturer
    Novagene
  • Backbone size w/o insert (bp) 5677
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Full length Pyrococcuas Horikoshii Leucyl-tRNA synthetase_WT
  • Alt name
    FL-Ph-LeuRS_WT
  • Species
    Full length Pyrococcuas Horikoshii Leucyl-tRNA synthetase
  • GenBank ID
    NC_000961.1
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHis tag (N terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning
  • 5′ sequencing primer catatgcatcaccatcaccatcacgcg
  • 3′ sequencing primer gctcagcttatcattcaataaaaatcgc
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-11a-pH-LeuRS_WT was a gift from Charles Carter (Addgene plasmid # 214193)
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