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pET28-His6-SUMO-Yes-SH2-AviTag
(Plasmid #214208)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214208 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pET28
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Yes kinase SH2 domain
  • Alt name
    Src-Homology 2 domain of Tyrosine-protein kinase Yes
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    324
  • Entrez Gene
    YES1 (a.k.a. HsT441, P61-YES, Yes, c-yes)
  • Promoter T7
  • Tags / Fusion Proteins
    • 6xHis (N terminal on backbone)
    • SUMO (N terminal on backbone)
    • AviTag (C terminal on backbone)

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2024.12.23.630085 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28-His6-SUMO-Yes-SH2-AviTag was a gift from Neel Shah (Addgene plasmid # 214208 ; http://n2t.net/addgene:214208 ; RRID:Addgene_214208)

  • For your References section:

    Accurate affinity models for SH2 domains from peptide binding assays and free-energy regression. Gagoski D, Rube HT, Rastogi C, Melo L, Li X, Voleti R, Shah NH, and Bussemaker HJ. Protein Science. 2025, 34:e70317. 10.1002/pro.70317
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