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Addgene

pMR-A-3-Sasg
(Plasmid #214349)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214349 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pCMV with a BR322 origin
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Spectinomycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    T7-RNAP
  • Species
    other

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMR-A-3-Sasg was a gift from Yongming Wang (Addgene plasmid # 214349 ; http://n2t.net/addgene:214349 ; RRID:Addgene_214349)

  • For your References section:

    Phage-assisted evolution of compact Cas9 variants targeting a simple NNG PAM. Qi T, Wang Y, Yang Y, Gao S, Liu J, Huang Q, Tian Y, Tang J, Zheng WV, Wang Y. Nat Chem Biol. 2023 Dec 5. doi: 10.1038/s41589-023-01481-5. 10.1038/s41589-023-01481-5 PubMed 38052959
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