SF-SON IRES Tomato
(Plasmid #214403)

• Purpose: Expresses human SOX10, OLIG2, NKX6.2 and tdTomato
• Depositing Lab: Tanja Kuhlmann
• Publication: Rapid and efficient generation of oligodendrocytes from human induced pluripotent stem cells using transcription factors. (unpublished)

Backbone

• Vector backbone: pRRL.SF-IRES-Pac
• Backbone size w/o insert (bp): 7812
• Total vector size (bp): 11275
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: SOX10, Olig2, NKX6.2
• Species: H. sapiens (human)
• Insert Size (bp): 3200
• Entrez Gene: SOX10 (a.k.a. DOM, PCWH, SOX-10, WS2E, WS4, WS4C)
• Entrez Gene: OLIG2 (a.k.a. BHLHB1, OLIGO2, PRKCBP2, RACK17, bHLHe19)
• Entrez Gene: NKX6-2 (a.k.a. GTX, NKX6.2, NKX6B, SPAX8)
• Promoter: SFFV

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: GTA ACG CCA TTT TGC AAG GCA TG
• 3′ sequencing primer: TCGACcgcccctctccctcc

Resource Information

• Supplemental Documents:
  • SFSONRFP20230914.plasmid_annotations.gbk

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry