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pMSCV-IRES-GFP/CALR ins5-KDEL
(Plasmid #214785)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214785 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pMSCV-IRES-GFP
  • Backbone manufacturer
    addgene(Plasmid #20672)
  • Backbone size w/o insert (bp) 6500
  • Vector type
    Retroviral

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    CALR ins5-KDEL
  • Species
    H. sapiens (human)
  • Entrez Gene
    CALR (a.k.a. CALR1, CRT, HEL-S-99n, RO, SSA, cC1qR)
  • Promoter MSCV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (unknown if destroyed)
  • 3′ cloning site XhoI (unknown if destroyed)
  • 5′ sequencing primer original
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMSCV-IRES-GFP/CALR ins5-KDEL was a gift from Norio Komatsu (Addgene plasmid # 214785 ; http://n2t.net/addgene:214785 ; RRID:Addgene_214785)

  • For your References section:

    Loss of KDEL function from a calreticulin frameshift mutation drives expression of an immature, mutant calreticulin-dependent form of the thrombopoietin receptor MPL. Masubuchi N, Yang Y, Imai M, Kihara Y, Morishita S, Edahiro Y, Komatsu N. Haematologica. 2025 Nov 6. doi: 10.3324/haematol.2025.287585. 10.3324/haematol.2025.287585 PubMed 41195513
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