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Addgene

pPBpuro-miRFP703-eDHFR(69K6)-mSOS1-linkercat
(Plasmid #214827)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214827 Standard format: Plasmid sent in bacteria as agar stab 1 $85
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Backbone

  • Vector backbone
    pPB
  • Backbone size w/o insert (bp) 6956
  • Total vector size (bp) 9938
  • Vector type
    Mammalian Expression
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    miRFP703-eDHFR(69K6)-mSos1-linkercat
  • Species
    Synthetic
  • Insert Size (bp)
    2981
  • Promoter CAG promoter
  • Tag / Fusion Protein
    • miRFP703 (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer none
  • 3′ sequencing primer none
    • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Dr. Shinya Tsukiji (Addgene plasmid # 172102: pPBpuro-mNG-eDHFR(69K6)-Tiam1)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPBpuro-miRFP703-eDHFR(69K6)-mSOS1-linkercat was a gift from Michiyuki Matsuda (Addgene plasmid # 214827 ; http://n2t.net/addgene:214827 ; RRID:Addgene_214827)

  • For your References section:

    Front-biased activation of Ras-Rab5-Rac1 loop coordinates collective cell migration. Jikko Y, Deguchi E, Matsuda K, Hino N, Tsukiji S, Matsuda M, Terai K. J Cell Sci. 2025 Jul 16:jcs.263779. doi: 10.1242/jcs.263779. 10.1242/jcs.263779 PubMed 40667649
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