pWPI_GW_BioID2_T2A_Blast
(Plasmid
#214831)
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Purpose(Empty Backbone) Destination vector for cloning BioID2 fusion proteins
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 214831 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 |
Backbone
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Vector backbonepWPI
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Backbone manufacturerAddgene #12254
- Backbone size (bp) 12742
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Vector typeMammalian Expression, Bacterial Expression, Lentiviral
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Selectable markersBlasticidin
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Tag
/ Fusion Protein
- BioID2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)ccdB Survival
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Copy numberHigh Copy
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2023.02.27.530345 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pWPI_GW_BioID2_T2A_Blast was a gift from Hani Goodarzi (Addgene plasmid # 214831 ; http://n2t.net/addgene:214831 ; RRID:Addgene_214831) -
For your References section:
Systematic identification of post-transcriptional regulatory modules. Khoroshkin M, Buyan A, Dodel M, Navickas A, Yu J, Trejo F, Doty A, Baratam R, Zhou S, Lee SB, Joshi T, Garcia K, Choi B, Miglani S, Subramanyam V, Modi H, Carpenter C, Markett D, Corces MR, Mardakheh FK, Kulakovskiy IV, Goodarzi H. Nat Commun. 2024 Sep 9;15(1):7872. doi: 10.1038/s41467-024-52215-7. 10.1038/s41467-024-52215-7 PubMed 39251607