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Addgene

pWPI_GW_BioID2_T2A_Blast
(Plasmid #214831)

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 214831 Standard format: Plasmid sent in bacteria as agar stab 1 $89

Backbone

  • Vector backbone
    pWPI
  • Backbone manufacturer
    Addgene #12254
  • Backbone size (bp) 12742
  • Vector type
    Mammalian Expression, Bacterial Expression, Lentiviral
  • Selectable markers
    Blasticidin
  • Tag / Fusion Protein
    • BioID2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2023.02.27.530345 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pWPI_GW_BioID2_T2A_Blast was a gift from Hani Goodarzi (Addgene plasmid # 214831 ; http://n2t.net/addgene:214831 ; RRID:Addgene_214831)
  • For your References section:

    Systematic identification of post-transcriptional regulatory modules. Khoroshkin M, Buyan A, Dodel M, Navickas A, Yu J, Trejo F, Doty A, Baratam R, Zhou S, Lee SB, Joshi T, Garcia K, Choi B, Miglani S, Subramanyam V, Modi H, Carpenter C, Markett D, Corces MR, Mardakheh FK, Kulakovskiy IV, Goodarzi H. Nat Commun. 2024 Sep 9;15(1):7872. doi: 10.1038/s41467-024-52215-7. 10.1038/s41467-024-52215-7 PubMed 39251607